mouse anti col2  (Chondrex Inc)

Journal: Cartilage

Article Title: Regenerative Variability of Human Juvenile Chondrocyte Sheets From Different Cell Donors in an Athymic Rat Knee Chondral Defect Model

doi: 10.1177/19476035241277946

Figure Lengend Snippet: Analysis of image stained with anti-type 2 collagen antibody using Image J. The area of the entire implanted cell sheet regenerative area ( A ); and the positive cell area ( B ). % COL2 area is shown as the percentage of ( B ) to ( A ). Bars: 500 μm.

Article Snippet: Polyclonal goat anti-COL1 (1:200, SouthernBiotech, Birmingham, USA), monoclonal mouse anti-COL2 (1:200, 2B1.5, ThermoFisher, USA), and monoclonal rabbit anti-hVIM (1:200, SP20, Abcam) were used as primary antibodies.

Techniques: Staining

Journal: Cartilage

Article Title: Regenerative Variability of Human Juvenile Chondrocyte Sheets From Different Cell Donors in an Athymic Rat Knee Chondral Defect Model

doi: 10.1177/19476035241277946

Figure Lengend Snippet: Scores of each system in all samples. The scores were arranged in order of modified O’Driscoll score. Bars sharing the same pattern represent samples from the same donor. Sample numbered 1, 3, and 5 represent the defect groups. ( A ) modified O’Driscoll score, ( B ) %COL1 area, ( C ) %COL2 area, and ( D ) COL1/2 ratios.

Article Snippet: Polyclonal goat anti-COL1 (1:200, SouthernBiotech, Birmingham, USA), monoclonal mouse anti-COL2 (1:200, 2B1.5, ThermoFisher, USA), and monoclonal rabbit anti-hVIM (1:200, SP20, Abcam) were used as primary antibodies.

Techniques: Modification

Journal: Cartilage

Article Title: Regenerative Variability of Human Juvenile Chondrocyte Sheets From Different Cell Donors in an Athymic Rat Knee Chondral Defect Model

doi: 10.1177/19476035241277946

Figure Lengend Snippet: Scatter plot of the correlation between modified O’Driscoll score and %COL 1 area ( A ), %COL 2 area ( B ), and COL2/1 ratio ( C ). Modified O’Driscoll score correlated significantly with each score (P<0.01).

Article Snippet: Polyclonal goat anti-COL1 (1:200, SouthernBiotech, Birmingham, USA), monoclonal mouse anti-COL2 (1:200, 2B1.5, ThermoFisher, USA), and monoclonal rabbit anti-hVIM (1:200, SP20, Abcam) were used as primary antibodies.

Techniques: Modification

Journal: Cartilage

Article Title: Regenerative Variability of Human Juvenile Chondrocyte Sheets From Different Cell Donors in an Athymic Rat Knee Chondral Defect Model

doi: 10.1177/19476035241277946

Figure Lengend Snippet: Comparison of each score between groups. ( A ) modified O’Driscoll score, ( B ) %COL1 area, ( C ) %COL2 area, ( D ) COL2/1 ratio. *<0.01, **<0.05.

Article Snippet: Polyclonal goat anti-COL1 (1:200, SouthernBiotech, Birmingham, USA), monoclonal mouse anti-COL2 (1:200, 2B1.5, ThermoFisher, USA), and monoclonal rabbit anti-hVIM (1:200, SP20, Abcam) were used as primary antibodies.

Techniques: Comparison, Modification